FluoPulse™ system
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FluoPulse™ system is the first commercial, plug-and-play Fluorescence Lifetime Fiber Photometry (FLiP) system available on the market, designed for neuroscience. This system measures changes in a biosensor's fluorescence lifetime, and is an essential tool to monitor intracellular biochemical reactions in the brains of freely moving animals.
The term "fluorescence lifetime" denotes the time lapse between the laser excitation and the release of fluorescence by the biosensor. Specially designed biosensors have unique lifetime distributions depending on whether they are in bound or unbound states. This disparity can be leveraged to study changes in neurophysiological states of key neural populations, including protein-protein interactions, changes in protein conformation, and the presence of neurotransmitters or other molecules of interest.
The FluoPulse™ system is designed to measure fluorescence lifetimes ranging between 1-10 ns and can resolve differences in lifetime of 10-20 ps. Thus, it is compatible with most biosensors designed for Fluorescence Lifetime Microscopy (FLIM) and Förster’s Resonance Energy Transfer (FRET), including FLIM-AKAR, GRAB-Ach3.0, etc.
The FluoPulse™ system includes:
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FLiP Cube contains optics, swappable bandpass and neutral density filters. It also contains excitation lasers and photodetectors for high temporal precision lifetime measurements. Users can customize the FLiP Cube according to experimental needs:
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Choose up to 3 excitation lasers: 406 nm, 450 nm, and 488 nm.
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Choose up to 2 detection windows: 500-560 nm and 600-700 nm.
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FLiP Console contains ultra-fast electronics for analog data sampling and 8 Digital Input/Output ports to synchronize behavioral measures. The FLiP Console is compatible with all configurations of the FLiP Cube.
The FluoPulse™ system uses a novel low-power Waveform Sampling method to measure the fluorescence lifetime in freely behaving animals. While traditional fluorescence lifetime measurement, Time-Correlated Single Photon Counting (TCSPC), is sensitive to background light that can skew experimental results, Waveform Sampling method is largely immune to background illumination interference. It is also possible to almost entirely cancel the effect of autofluorescence originating from fiber patch cords. Both immunities are crucial for in-vivo lifetime measurements, where fiber optic patch cords are required and ambient light cannot be eliminated. In addition, since Waveform Sampling collects many more photons per laser excitation, it provides a higher sampling rate and precision than the traditional method.
Furthermore, fluorescence lifetime photometry measurements offer several advantages compared to classic, intensity-based photometry. Since lifetime is a molecular property, it is less susceptible to issues like photobleaching, expression levels, excitation power, and movement artifacts, which commonly pose challenges in intensity-based photometry. Consequently, fluorescence lifetime measures are more consistent between different animals and are exceptionally reliable for prolonged experimental paradigms spanning weeks and months, a critical timeframe for clinical studies.
Thus, the FluoPulse™ system provides a state-of-the-art tool to measure fluorescence lifetime in a freely-moving animal, and opens avenues for discoveries in neuroscience and other fields where fluorescence lifetime offers valuable insights into the sample under investigation.
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The FluoPulse system includes :
- FLiP Console, the core of the system
- FLiP Cube with built-in laser diodes with their drivers, and integrated high-speed detectors with their amplifiers
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Doric Neuroscience Studio software
- All necessary cables and an output fiber optic patch cord
Optional items:
- Pigtailed 1x1 Fiber-optic Rotary Joint with Holder (fixed and gimbal)
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Optical Power Meter to monitor and calibrate excitation power
- Workstation
- danse™ analyzing software
Accessories:
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Low-autofluorescence Optical Patch Cords bring light to an implanted cannula
- Fiber-optic Cannulas for chronic implant
- Mating sleeves for the ferrule-to-ferrule interconnect
Note: Other light sources and fluorophore combinations are possible.
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Lifetime range 1 - 10 ns Lifetime resolution 10 - 20 ps Sampling Rate 10 Hz Excitation Pulse Width 300 ps Peak power:
- 406 nm
- 450 nm
- 488 nm
20 mW
80 mW
50mWPulse Repetition Rate 700 kHz *
* depend on the number of lasers -
Drawing Fluorescence Lifetime Photometry Cube
Fluorescence Lifetime Photometry Console
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