6 ports Fluorescence Mini Cube - GCaMP and Red fluorophore

6 ports Fluorescence Mini Cube - GCaMP and Red fluorophore
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  • This cube detects the fluorescence from two calcium indicators and GCaMP isosbestic point. The separation of functional and isosbestic fluorescence signals of GCaMP is possible with modulated light sources. E1 and E2 stand for excitation at the cube's schematic, IE for isosbestic excitation, F1 and F2 for respective fluorescence windows, and S for the sample port.

    Compared to Fluorescence Mini Cubes with only FC connectors on all ports, the ones with integrated photodetectors provide a 100% better signal-to-noise ratio (30% signal increase and a 40% noise reduction). Moreover, in the latest generation of Mini Cubes, LEDs and LED drivers are integrated as well to reduce the number of connections and the system’s overall footprint. In his generation, it is also possible to replace the excitation filter (to adjust the isosbestic excitation from 405 nm to 410 nm for instance) and decrease the excitation power by adding attenuating filters (for low power photometry).

  • Mini Cube
    Wavelength range 350 to 1100 nm
    Optical fiber compatibility - Core diameter 200 or 400 µm
    - NA 0.37 to 0.57
    Spectral bandwidth

    GCaMP :
    - Isosbestic Excitation 400-410 nm
    - Excitation 460-490 nm
    - Emission 500-540 nm

    Red Fluorophore :
    - Excitation 540-570 nm
    - Emission 580-680 nm

    Optical filter attenuation > OD 5 outside band
    Dimensions - FMC: 101 x 59 x 26 mm
    - iFMC: 108 x 92 x 50 mm
    - ilFMC: 152 x 109 x 52 mm
    Built-in Detectors iFMC ilFMC
    Detector Sensitivity - 0.38 A/W @ 550 nm
    - 0.60 A/W @ 960 nm (peak)
    - 0.38 A/W @ 550 nm
    - 0.60 A/W @ 960 nm (peak)
    Amplification 3 levels : 1x, 10x, 100x 2 levels : 1x, 10x
    Transimpedance gain - 2.0 E09 V / A (1x)
    - 2.0 E10 V / A (10x)
    - 2.0 E11 V / A (100x)
    - 2.0 E09 V / A (1x)
    - 2.0 E10 V / A (10x)
    Conversion gain
    @ 550 nm
    - 0.76 V / nW (1x)
    - 7.60 V / nW (10x)
    - 76.0 V / nW (100x)
    - 0.76 V / nW (1x)
    - 7.60 V / nW (10x)
    Saturation level
    @ 550 nm
    - 7.2 nW (1x)
    - 0.72 nW (10x)
    - 72 pW (100x)
    - 7.2 nW (1x)
    - 0.72 nW (10x)
    NEP < 12 fW/√Hz < 12 fW/√Hz
    Bandwidth - DC : 0 - 1000 Hz
    - AC : 30 - 1000 Hz
    - DC : 0 - 1000 Hz
    - AC : 30 - 1000 Hz
    Electrical interface BNC BNC
    Output Voltage 0.0 to 5.5 V 0.0 to 5.5 V
    Output impedance 50 Ω 50 Ω
    Sensor type Silicon photodiode Silicon photodiode
    Sensor Area 1 x 1 mm 1 x 1 mm
    Input voltage 12 V DC (power supply included) 12 V DC (power supply included)
    Built-in LEDs (ilFMC)
    Max Current 500 mA
    Emitter size 400  µm diameter - NA0.22
    Max Output Power
    (fibre 400 µm)
    LED 400-410 nm
    = 130 µW (at 200 mA)
    LED 460-490 nm
    = 130 µW (at 200 mA)
    LED 540-570 nm
    = 130 µW (at 200 mA)
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