{"title":"Basic Fiber Photometry (FMC)","description":"\u003cdiv id=\"DoricJustify\"\u003e\n\u003cp\u003eIn fiber photometry experiments, fluorescent sensors are used to monitor neurotransmitter levels or neural activity. Doric Fluorescence Mini Cube (FMC) system combines excitation lights, high sensitivity photodiode light detectors, and all filtering and coupling optics to record fiber-photometry signals.  In some configurations, it adds the optogenetics stimulation port.\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"\/fr\/pages\/fiber-photometry\"\u003e← Back to Fiber Photometry\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e","products":[{"product_id":"fmc7-gcamp","title":"7 ports Fluorescence Mini Cube - GCaMP","description":"\u003cp\u003eThis cube combines the detection of two calcium indicators (GCaMP and red fluorophores) with the activation of red opsins. It integrates dichroic mirrors to combine fluorophores excitations, isolate the fluorescence emissions, and let opsin stimulation light through. At the same time, narrow bandpass filters limit the excitation and detection spectral bands:\u003c\/p\u003e\n","brand":"Doric Lenses Inc.","offers":[{"title":"Default Title","offer_id":47933647323378,"sku":null,"price":0.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1916\/0562\/files\/ILFMC7-G3_PERSPECTIVE_FRONT.png?v=1757428140"},{"product_id":"neuroscience-console-500","title":"Neuroscience Console 500","description":"\u003cp\u003e\u003cspan\u003eNeuroscience Console 500 (NC500) is Doric Lenses' latest and most advanced data acquisition system. It's designed to handle multiple experimental techniques at once, with faster sampling rates and higher resolution than any previous version.\u003cbr\u003e\u003cbr\u003eNC500 works seamlessly with the new \u003cstrong\u003eDoric Neuroscience Studio (version 6)\u003c\/strong\u003e, which offers an easy-to-use and efficient interface for collecting and visualizing all your data in one place.\u003c\/span\u003e\u003c\/p\u003e","brand":"Doric Lenses Inc.","offers":[{"title":"Default Title","offer_id":47933649518834,"sku":null,"price":0.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1916\/0562\/files\/DNS_console_FRONT_VIEW_UPDATE.png?v=1775590794"},{"product_id":"fiber-photometry-system-basic","title":"Basic Fiber Photometry System","description":"\u003cp\u003eThe conventional fiber photometry systems for behaving animals consist of Fiber Photometry Console (FPC), an integrated Fluorescence Mini Cube (FMC), Pigtailed 1x1 Fiber-optic Rotary Joint (FRJ_1x1_pt), and Mono Fiber-optic Cannula (MFC). The fiber-optic rotary joint transfers the light signals and allows fiber tether rotation. Currently, we offer LED light sources for GCaMP (or other green fluorophores), GCaMP with Red Fluo, and FRET(CFP\/YFP). Explore the possibility of customization with other light sources, fluorophores, or assisted rotary joint.\u003cbr\u003e\u003c\/p\u003e\n","brand":"Doric Lenses Inc.","offers":[{"title":"Default Title","offer_id":47933653844210,"sku":null,"price":0.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1916\/0562\/files\/Basic_system_c8028e54-9244-4b85-addf-cd81ba4f20f0.png?v=1769715127"},{"product_id":"fmc6","title":"6 ports Fluorescence Mini Cube - GCaMP and Red fluorophore","description":"\u003cp\u003eThis cube detects the fluorescence from two calcium indicators and GCaMP isosbestic point. The separation of functional and isosbestic fluorescence signals of GCaMP is possible with modulated light sources. E1 and E2 stand for excitation at the cube's schematic, IE for isosbestic excitation, F1 and F2 for respective fluorescence windows, and S for the sample port.\u003c\/p\u003e\n\u003cp\u003eCompared to Fluorescence Mini Cubes with only FC connectors on all ports, the ones with integrated photodetectors provide a 100% better signal-to-noise ratio (30% signal increase and a 40% noise reduction). Moreover, in the latest generation of Mini Cubes, LEDs and LED drivers are integrated as well to reduce the number of connections and the system’s overall footprint. In his generation, it is also possible to replace the excitation filter (to adjust the isosbestic excitation from 405 nm to 410 nm for instance) and decrease the excitation power by adding attenuating filters (for low power photometry).\u003c\/p\u003e","brand":"Doric Lenses Inc.","offers":[{"title":"Default Title","offer_id":47933655810290,"sku":null,"price":0.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1916\/0562\/files\/ILFMC6.jpg?v=1757428537"},{"product_id":"fmc5-gcamp-og","title":"5 ports Fluorescence Mini Cube - GCaMP and Optogenetic stimulation","description":"\u003cp\u003eDual excitation band fiber photometry combined with optogenetics use a Fluorescence Mini Cube with 5 ports: one for 460-490 nm GCaMP excitation, one for 405, 415, 425 or 440 nm isosbestic excitation, one for 500-540 nm window of isosbestic and functional fluorescence detection, one for 580-680 nm opsin silencing light, and one for the sample under the test. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations, isolate the fluorescence emission, and let opsin stimulation light through. At the same time, narrow bandpass filters limit the excitation fluorescence spectrum.\u003c\/p\u003e","brand":"Doric Lenses Inc.","offers":[{"title":"Default Title","offer_id":47933655843058,"sku":null,"price":0.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1916\/0562\/files\/ILFMC5-G3_PERSPECTIVE_FRONT.png?v=1757428541"},{"product_id":"fmc4-fret","title":"4 ports Fluorescence Mini Cube - FRET","description":"\u003cp\u003eFRET YFP-CFP recordings start with the donor fluorophore excitation with a 420-445 nm wavelength band. The excited donor fluorophores lose part of that energy to fluorescence in the 460-500 nm band. The rest goes to acceptor fluorophores in a distance-dependent, radiationless manner. The acceptor then emits the fluorescence in the 528-556 nm window.\u003c\/p\u003e","brand":"Doric Lenses Inc.","offers":[{"title":"Default Title","offer_id":47933655908594,"sku":null,"price":0.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1916\/0562\/files\/BuiltInLEDandDetector_480_94fe8f79-84ac-4c58-a1cb-6cffd4cc9185.jpg?v=1757428545"},{"product_id":"fmc4-gcamp","title":"4 ports Fluorescence Mini Cube - GCaMP","description":"\u003cp\u003eDual excitation band fiber photometry measurements use a Fluorescence Mini Cube with 4 ports: one port for the functional fluorescence excitation light, one for the isosbestic excitation, one for the fluorescence detection, and one for the sample. The cube has dichroic mirrors to combine isosbestic and fluorescence excitations and separate the fluorescence emission and narrow bandpass filters limiting the excitation fluorescence spectrum. E stands for fluorescence excitation, IE for isosbestic excitation, F for fluorescence, and S for the sample port on the cube's schematic. The separation of functional and isosbestic fluorescence signals of GCaMP is possible with modulated light sources.\u003c\/p\u003e\n\u003cp\u003eCompared to Fluorescence Mini Cubes with only FC connectors on all ports, the ones with integrated photodetectors provide a 100% better signal-to-noise ratio (30% signal increase and a 40% noise reduction). \u003c\/p\u003e\n\u003cp\u003eMoreover, in the latest generation of Mini Cubes, LEDs and LED drivers are integrated as well to reduce the number of connections and the system’s overall footprint. In his generation, it is also possible to replace the excitation filter and decrease the excitation power by adding attenuating filters (for low power photometry). \u003c\/p\u003e","brand":"Doric Lenses Inc.","offers":[{"title":"Default Title","offer_id":47933655974130,"sku":null,"price":0.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1916\/0562\/files\/ILFMC4_a2234272-fc25-4ab7-9605-c45210e909e6.png?v=1757428547"},{"product_id":"fmc4-fl-og","title":"4 ports Fluorescence Mini Cube - Single fluorophore with Optogenetics stimulation","description":"\u003cp\u003eThis cube is for measurements involving excitation, optogenetic activation\/silencing, fluorescence detection, and sample ports. Such a cube can be used for green fluorescence measurements combined with the activation of red opsins in the 580-650 nm band.\u003c\/p\u003e","brand":"Doric Lenses Inc.","offers":[{"title":"Default Title","offer_id":47933656006898,"sku":null,"price":0.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1916\/0562\/files\/ilFMC4-G2_GFP-Opsin-2.png?v=1757428550"},{"product_id":"fmc3","title":"3 ports Fluorescence Mini Cube - Single Fluorophore","description":"\u003cp\u003eSingle excitation band fiber photometry measurements use an FMC with 3 ports: one port for the excitation light, one for the fluorescence detection, and one to connect to the sample. The cube has a dichroic mirror to let the excitation light through and separate the fluorescence emission and narrow bandpass filters to limit the excitation or fluorescence spectrum. E stands for fluorescence excitation, F for fluorescence, and S for the sample port on the cube's schematic. Currently, only Green Fluorophore (GFP-like) configurations or Red Fluorophore (RFP-like) are offered. Still, the filter set can be customized for other fluorophores on request.\u003c\/p\u003e","brand":"Doric Lenses Inc.","offers":[{"title":"Default Title","offer_id":47933656039666,"sku":null,"price":0.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1916\/0562\/files\/BuiltInLEDandDetector_480_b307d70e-b437-4178-b523-f9123257f349.jpg?v=1757428552"},{"product_id":"fiber-photometry-console","title":"Fiber Photometry Console","description":"\u003cp\u003eThis FPGA-based data acquisition unit synchronizes the output control and the input data acquisition. It seamlessly integrates with Doric Neuroscience Studio that provides a user interface for multi-channel photometry experiments. The software interface enables control over the CW pulsed excitation light, or sinusoidal waveform trig of an external source (i.e., LED driver) with 4 TTL and 4 analog voltage outputs. The software interface displays real-time recording data of up to 4 detector input signals. Signal processing does basic \u003cb\u003earithmetic\u003c\/b\u003e functions between two channels, \u003cb\u003efilter\u003c\/b\u003e function to remove unwanted frequencies from the data, calculates \u003cb\u003eΔF\/F\u003c\/b\u003e, etc. New functionalities are being continuously added.\u003c\/p\u003e","brand":"Doric Lenses Inc.","offers":[{"title":"Default Title","offer_id":47933656072434,"sku":null,"price":0.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1916\/0562\/files\/fiber-photometry-console.jpg?v=1757428553"},{"product_id":"isosbestic-led-filter-set-for-ilfmc-g3","title":"Isosbestic LED","description":"\u003cp data-pm-slice=\"1 1 []\"\u003eThis isosbestic LED (ILED) can be swapped to change the isosbestic excitation of an existing integrated Fluorescence Mini Cube (ilFMC-G3) or the Rotary Bundle-imaging Fluorescence Mini Cube (RBFMC-G2), respectively. The ILED provides a simple solution to expand to new biosensors. The iLED comes in four possible options: 405 nm, 415 nm, 425 nm, and 440nm. Each ILED comes with a bandpass filter that narrows the excitation wavelength to a ~10nm bandwidth.\u003c\/p\u003e","brand":"Doric Lenses","offers":[{"title":"Default Title","offer_id":48901388959986,"sku":null,"price":0.0,"currency_code":"CAD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1916\/0562\/files\/Isosbestic_LED_block.png?v=1774355727"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0771\/1916\/0562\/collections\/image-7.png?v=1769718920","url":"https:\/\/neuro.doriclenses.com\/fr\/collections\/classic-fiber-photometry.oembed","provider":"Doric Lenses","version":"1.0","type":"link"}