5 ports Fluorescence Mini Cube - Two Fluorophores
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Two excitations and two detections require a 5 ports FMC optimized for the maximum transmission of detected fluorescence, the minimal cross-talk of the two fluorescence spectra, and the highest possible isolation of the detection ports from the excitation light. We currently offer Green Fluorophore (GFP-like) configurations and Red Fluorophore (RFP-like). Still, the filter set can be customized for other fluorophores on request.
Compared to Fluorescence Mini Cubes with only FC connectors on all ports, the ones with integrated photodetectors provide a 100% better signal-to-noise ratio (30% signal increase and a 40% noise reduction). Moreover, the versions with integrated LEDs reduce the number of optical connections and overall system footprint.
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Mini Cube Wavelength range 350 to 1100 nm Optical fiber compatibility - Core diameter 200 or 400 µm
- NA 0.37 to 0.57Spectral bandwidth Green Fluorophore :
- Excitation 460-490 nm
- Emission 500-550 nm
Red Fluorophore :
- Excitation 540-570 nm
- Emission 580-680 nmOptical filter attenuation > OD 5 outside band Dimensions 57 mm x 63 mm x 26 mm Built-in Detectors Detector Sensitivity - 0.38 A/W @ 550 nm
- 0.60 A/W @ 960 nm (peak)
Amplification 3 levels : 1x, 10x, 100x Transimpedance gain - 2.0 E09 V / A (1x)
- 2.0 E10 V / A (10x)
- 2.0 E11 V / A (100x)Conversion gain
@ 550 nm- 0.76 V / nW (1x)
- 7.60 V / nW (10x)
- 76.0 V / nW (100x)Saturation level
@ 550 nm- 7.2 nW (1x)
- 0.72 nW (10x)
- 72 pW (100x)NEP < 12 fW/√Hz Bandwidth - DC : 0 - 1000 Hz
- AC : 30 - 1000 HzElectrical interface BNC Output Voltage 0.0 to 5.5 V Output impedance 50 Ω Sensor type Silicon photodiode Sensor Area 1 x 1 mm Input voltage 12 V DC (power supply included) Built-in LEDs Max Current 500 mA Emitter size 400 µm diameter - NA0.22 Max Output Power
(fibre 400 µm)LED 460-490 nm
= 1.5 to 120 µW (adjustable)LED 540-570 nm
= 50 µW (fixed)
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